Procedures
Experiment 1
1-1 Capsule
melting time/ Room temp(81.6F/ph7.0 (water))
1) Condition : room
temperature-81.6F, tap water-PH 7.0, 6.73g in the plastic container
2) Put 3 plastic
containers with the same mass of water. Next, add one capsule to each
container: 1 expired 5 billion probiotics, 1 new 5 billion probiotics , and 1
90 billion probiotics capsule.
3) Observe each for 5
minutes to observe any transitions of capsules. (enteric coated capsule has two
layers: white part and a clear inner
part)
4) Write down the changing
room temperature for each stage
5) Cracking time is
more important than when it completely dissolves because when the capsule
cracks, probiotics spurt out into the liquid.
6) Continue until the
capsules dissolve completely in the water
7) Take photos each
stage for recording
1) Condition: tap
water-PH7.0, 6.73g in the plastic container, 5billion probiotics capsule, and boiled
water to refill the water in order to maintain the body temperature for each
stage.
2) Keep in mind! Measuring
the temperature is important. Start the temperature at 98.6
°F. This experiment is an independent variable of the stomach’s environment (the
average internal temperature is 37.0
°C (98.6 °F)).
An error value of 0.1~0.5 will be allowed because it
can be assumed the body temperature is different for each person.
3) Observe for
3minutes for the transition of the capsule (in this case, condition 3minutes
gap because it expects fast dissolving time).
4) Before observing transition
of the capsule for each stage, measure the temperature of water and add water
with the eyedropper and keep refilling warm water until body temperature.
5) Mark the breaking
capsule time. Continue to observe transition until dissolved completely.
1-3 5 billion Capsule Melting Time in
ph2.1/ph2.2(room temperature81.5F)
1) Condition: room
temperature-81.6F, lemon extract(PH2.1) 6.73g, vinegar(PH2.2) 6.73g , and 2, 5billion
enteric coated capsule
2) Measure the PH
with a PH tester for the lemon extract and vinegar.
3) Put one container
with vinegar and the other with lemon extract, put them at the same time.
4) Observe the stage
every 30 minutes during 3 hours.
5) Check what phase
the capsule is in and write down.
1-4 Capsule
Melting Time Experiment (Body Temperature
Average 98.6F Apple Vinegar PH 2.4) 5billion
Probiotics
1) Condition: body temperature
98.6F(±0.4F), apple vinegar (PH2.4) 6.73g, heated vinegar
100g, 1- 5billion enteric coated capsule, eye dropper, digital thermometer, 1
small plastic bowl, clock
2) Note: It is very important to keep the body temperature the same. If you
are able to keep the same temperature (98.6F), it will have a perfect result.
If you don’t have special machine like me, use an eye dropper and add more
heated vinegar whenever the temperature falls below 98.4F (nearly every 10-15
seconds).
3) Prepare
6.73g apple vinegar in the plastic container.
4) Add 1 5billion probiotics enteric coated capsule.
5) Record every temperature, and observe phase of the capsule
6) Cover the container with warm cloth.
7) Check and write down during those 3hours.
1-5 Capsule
Melting Time Experiment (body temperature
98.6F, Apple Vinegar PH2.4) 90 billion probiotics
1) Keep temperature at
98.6F with heated vinegar, apple vinegar PH2.4
500g, one plastic container, thermometer, clock,
90 billion probiotics
capsule, eye dropper, clock
2) Prepare
6.73g apple vinegar in the container. Add 1 90 billion probiotics capsule.
3) Heat apple vinegar on the stove top. Pour the
container, and measure the temperature until it is 98.6F.
4) Every 20-30 seconds, observe the temperature, and
add some heated vinegar with eye dropper.
5) Watch the transition of the capsule.
6) Record every stage that change.
Experiment2
Probiotics’
stability in a body temperature environment
4 tester tubes, 4-6.4g water with expired 5 billion
probiotics capsule, new 5 billion probiotics capsule, new 90 billion probiotics
capsule, Kimchi juice, microscope slide, cover glass, eye dropper, 1 bottle
Immersion oil type A, 40swabs, alcohol for cleansing the slides
1) Fill 6.4g of water
in 3 tester tubes, fill 6.4g Kimchi juice in the tester tube.
2) Break 3 capsules
gather only powder of probiotics, Add different subjects in the tester tubes.
3) To keep the body temperature(98.6F), grab
4tester tubes with left hands. Our body temperature is 98.6F, but the body skin
temperature is about 93.2F, but this temperature is also the probiotics’ growth
temperature. When you have to use left hand you can keep the tubes between belt
of your pants and skin of waist.
4) Observe the status
of probiotics sample from tube with swab. Rub with a swab some probiotics with
water on the surface of the microscope
slide, and cover with cover glass.(write down their name tag on the slides)
5) After watching the
microscope, clean up the slides and microscope lenses of every stage.
6) Use your
microscope. From 40x lens to 1000X observe the activities of every subject. (1000X
lens need immersion oil)
7) Note: For metric
transfer, according to the degree you can make score 1-10.
8) During your sleep
time, you can cover the tubes by throwing several layers of clothing.
9) Observe Until
stage12.(30hours)
Experiment 3
Survival of Probiotics
in the pH2(vinegar)
1) In the middle of
stage 4or 5, collect probiotics(90billion) with moisture from the tube.
2) Drop the
probiotics on the microscope slide.
3) Add one drop of
vinegar(use eye dropper)
4) Observe the slide
from 40X to 1000X
5) Record the probiotics’
activity and disappearing time.
Note: This experiment can analogize the
stomach environment.
Experiment 4
Observation of
Direct Antagonism
1) During experiment 2 stage 4, fill one drop of 90
billion probiotics onto another microscope slide.
2) Add a sample of moldy
bread, orange, and/or onions.
3) Observe the reaction
and activities of probiotics through the microscope.
Observation of
Direct Antagonism (Experiment 4)
(probiotics, moldy bread, moldy orange, onion black fungi : Aspergillus
niger)
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